SOLIDEX®-ISOEx 人NK细胞分离试剂盒(柱式,Untouched)
订购信息
| 产品货号 | 产品名称 | 规格 | 价格 (USD) |
|---|---|---|---|
| GM-NK-Cell-iso-kit-10T | SOLIDEX®-ISOEx 人NK细胞分离试剂盒(柱式,Untouched) | 10 Tests | $190 |
| GM-NK-Cell-iso-kit-50T | SOLIDEX®-ISOEx 人NK细胞分离试剂盒(柱式,Untouched) | 50 Tests | $570 |
| GM-NK-Cell-iso-kit-100T | SOLIDEX®-ISOEx 人NK细胞分离试剂盒(柱式,Untouched) | 100 Tests | $1004 |
| GM-NK-Cell-iso-kit-750T | SOLIDEX®-ISOEx 人NK细胞分离试剂盒(柱式,Untouched) | 750 Tests |
产品规格
- 目标细胞群 NK细胞
- 标记类型 Indirect
- 磁珠类型 Non-releasable
- 分选方式 柱式
- 分离方法 Negative NK Cell Isolation (Untouched)
- 储存条件 2℃ - 8℃ 保存
产品详情
| 产品货号 | GM-NK-Cell-iso-kit |
| 产品名称 | SOLIDEX®-ISOEx 人NK细胞分离试剂盒(柱式,Untouched) |
| 英文名称 | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) |
| 产品详情 | SOLIDEX®-ISOEx 人NK细胞分离试剂盒(柱式,Untouched)是一种间接NK细胞分离试剂盒(柱式,不可释放) |
| 应用详情 | SOLIDEX®-ISOEx 人NK细胞分离试剂盒(柱式,Untouched)用于阴性NK细胞分离(Untouched),其主要特点包括:保留细胞天然功能;维持表面标志物完整性;适用于功能研究。 |
| 组分 |
|
| 标记类型 | Indirect |
| 磁珠类型 | Non-releasable |
| 分选方式 | 柱式 |
| 关键特性 | 保留原生细胞功能;保持表面标记的完整性;非常适合功能研究 |
| 目标细胞群 | NK细胞 |
| 储存条件 | 2℃ - 8℃ 保存 |
应用详情
实验方案
- 准备:在分离缓冲液中重悬外周血单个核细胞。
- 标记:加入分离鸡尾酒,在2-8°C下孵育15分钟(3)。
- 洗涤:用缓冲液洗涤细胞以去除未结合的抗体(4)。
- 磁珠孵育:加入抗生物素纳米磁珠,在2-8°C下孵育15分钟(5)。
- 分离:将悬浮液通过LS柱;收集流出液(富集的NK细胞)(6)。
验证数据
Efficient Isolation of NK Cells Using SOLIDEX®-ISOEx Untouched 人NK细胞 Isolation Kit (柱式)
产品货号GM-NK-Cell-iso-kit
To determine the purity of isolated NK cells, CD56+ NK cells were magnetically sorted from human peripheral blood mononuclear cells (PBMCs). Cells before and after separation were stained with CD3-APC and CD56-PE antibodies and subsequently analyzed by flow cytometry. Results demonstrated a remarkable increase in CD56+ NK cell purity from 8.19% pre-isolated to 94.3% post-isolated, confirming highly effective NK cell enrichment.
Figure 5. Efficient Isolation of NK Cells Using SOLIDEX®-ISOEx Untouched 人NK细胞 Isolation Kit (柱式). The non-NK cells were labeled with biotinylated antibodies. After incubating with anti-biotin magnetic nanobeads, these cells were depleted by cell isolation column. The NK cells were harvested by collecting the flowthrough component. The NK cells in pre-isolated human PBMCs. NK cells with high purity were enriched.
High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched 人NK细胞 Isolation Kit (柱式)
产品货号GM-Tg-hg-NK-Cell-iso-kit
Human peripheral blood mononuclear cells (PBMCs) were freshly recovered and subjected to NK cell isolation using SOLIDEX®-ISOEx Untouched 人NK细胞 Isolation Kit (柱式) and Company M’s NK Cell kit. The isolated cell population was analyzed by flow cytometry to evaluate purity and recovery rate. SOLIDEX®-ISOEx Untouched 人NK细胞 Isolation Kit (柱式) provides superior performance in isolating NK cells from human PBMCs, delivering high purity and recovery rate for downstream applications.
Figure 6. High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched 人NK细胞 Isolation Kit (柱式). The flow cytometry analysis of the isolated cells from cryopreserved PBMCs.
背景介绍
为什么选择SOLIDEX®-ISOEx?
- Untouched的纯度:提供高纯度(验证数据显示从约8%提升至>94%),同时保持NK细胞无标记。
- 保留功能:阴性选择策略确保零激活,维持细胞的天然杀伤能力。
- 柱式效率:柱子放大磁场,与无柱方法相比,确保非靶细胞的卓越 depletion。
- 快速工作流程:优化的方案具有短孵育时间,可实现快速分离。
常见问题
样本有哪些要求?我可以使用冷冻的PBMC吗?
需要更多产品数据或案例?
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